The activity of an Enzyme is affected by its environmental conditions. Changing these alter the rate of reaction caused by the enzyme. In nature, organisms adjust the conditions of their enzymes to produce an Optimum rate of reaction, where necessary, or they may have enzymes which are adapted to function well in extreme conditions where they live.
During practical sessions you have already determined the effect of pH and temperature. In the following. Throughout this section we will be investigating the effect of substrate and enzyme concentration of the rate at which a reaction occurs.
Effects of substrate concentration on enzyme activity
The substrate is the molecule that a reaction.
Increasing the substrate concentration the rate of reaction. This is because more substrate molecules will be with enzyme molecules, so more product will be formed.
However, after a certain concentration any increase will have effect on the rate of reaction, since concentration will no longer be the factor. The enzymes will effectively become , and will be working at their maximum possible rate. The rate of reaction will not be able to increase any further.
Effect of substrate concentration answers
- Increasing Substrate Concentration increases the rate of reaction. This is because more substrate molecules will be colliding with enzyme molecules, so more product will be formed.
- However, after a certain concentration, any increase will have no effect on the rate of reaction, since Substrate Concentration will no longer be the limiting factor. The enzymes will effectively become saturated, and will be working at their maximum possible rate
Effect of enzyme concentration on enzyme activity
As enzyme concentration increasesrate of reaction will increase
More enzymes meansmore collisions with substrate molecules
will only have an effect up to a certain concentration asthere is only a certain number of substrate molecules present