Biochemistry and Microbiology Remedial Action

During preparation for external examination, it has been found that there are a few pieces of information missing from your assignments.  The following virtual learning classes have been created to allow you to complete the remedial tasks required, as discussed by your tutor.

Factors effecting enzyme concentration

Effects of substrate concentration on enzyme activity

The substrate is the molecule that

reacts inis formed by
a reaction.

Increasing the substrate concentration

decreasesincreasesdoes not effect
the rate of reaction. This is because more substrate molecules will be
jumping aroundcollidingstopping
 with enzyme molecules, so more product will be formed.

However, after a certain concentration any increase will have

a lota littelNo
 effect on the rate of reaction, since 
 concentration will no longer be the
 factor. The enzymes will effectively become
, and will be working at their maximum possible rate. The rate of reaction will not be able to increase any further.

Effect of enzyme concentration on enzyme activity

Сombine elements from the left column with the elements on the right
As enzyme concentration increases
rate of reaction will increase
More enzymes means
there is only a certain number of substrate molecules present
will only have an effect up to a certain concentration as
more collisions with substrate molecules

COSHH regulation

Adhering to COSHH regulations

In order to adhere to COSHH regulations when working with micro organisms there are 8 steps that must be taken. Can you put these in the correct order
Ensure that control measures are used and maintained properly
Monitor the exposure to the micro-organism
Carry out appropriate health surveillance where necessary
Ensure employees are properly informed, trained and supervised
Decide what precautions are needed
prevent or adequately control exposure to the micro-organism
prepare plans and procedures to deal with accidents, incidents and emergencies
Asses the risks to health arising from using tis micro-organism

hazards when working with specific micro organisms

There are many types of micro-organisms, each with their own individual hazards. However we will be concentrating on only 5 types: Salmonella species, vibrio cholera, mycobacterium tuberculosis, plasmodium species and HIV.


Salmonella - There are 6 different subtypes of salmonella each of which are

to humans.  Human infection usually occurs when consuming contaminated food and water, contact with infected faeces, as well as contact with infective animals, animal feed or other infected humans. Salmonella can cause 4 different clinical manifestations